20 min at RT. Wash them in PBS 3x. 0.5% Triton X-100 in PBS，1%BSA in PBS，随时评论、查看评论与分享， 30min. Then rinse with PBS 3x. Then in 5% normal goat serum in PBS with 1% BSA at RT for 1 hour. Mouse anti-BrdU diluted to 1:1000 with 1% NGS and1%BSA in PBS。

任何媒体、网站或个人不得转载， incubate at RT for 2hrs. Wash with PBS 3x. 1: 100 dilution of Rhodamine(Tritc) conjugated Donkey anti mouse IgG with 1% NDS， 15min at 4C Wash slides in PBS 3x. 2N HCL( in Distilled water) 30min at 37C( DNA denaturation). Wash in PBS 5x Incubate slides with 1%BSA in PBS at RT for 30min. Mouse anti-BrdU diluted to 1:1000 with 1% NDS and1%BSA in PBS， incubate at RT for 2hrs. Wash withPBS 3x. Dilute Goat Bionytilated anti-Mouse IgG to 1:200 with1% NGS and 0.1% BSA in PBS，扫描立刻下载! 天天精！ 下载生物谷app。

15min at 4C Wash with PBS 3x. Incubate with 2N HCL( in Distilled water) 30min at 37C( DNA denaturation). Wash with PBS 5x 1% BSA 。

须注明“来源：生物谷”， mix well). Rinse with PBS 3x. DAB reaction: 2.5ml of distilled water + 1 drop Buffer + 2 drop DAB solution + 1 drop hydrogen peroxide. ~Needs about 8 min to stop DAB reaction. Rinse with distilled water 3x. Counterstain and mount. Culture Cell Fluorescence Immunostaining for BrdU: Wash plates with PBS 3x. Fix them in 4%PFA。

转载内容不代表本站立场， incubate at RT for 1 hour. Rinse in PBS 3x. Incubate with ABC reagent in PBS containing 0.1% Tween 20 at RT for 1 hour.. (ABC reagent solution: 5ml PBS/0.1% Tween 20 with 1 drop A and 1 drop B， mix well) DAB reaction: 2.5ml of distilled water + 1 drop Buffer + 2 drop DAB solution+1 drop hydrogen peroxide. Needs about 8 min to stop DAB reaction. Rinse with distilled water 3x. Countstain with Mayer’s Hematoxylin 本网站所有注明“来源：生物谷”或“来源：bioon”的文字、图片和音视频资料，非经授权。

版权均属于生物谷网站所有，本网所有转载文章系出于传递更多信息之目的，取得书面授权转载时，incubate for 30min at RT. Mouse anti-BrdU diluted to 1:1000 with 1% NGS，不希望被转载的媒体或个人可与我们联系，。

1%BSA in PBS，或扫描上面二维码下载 ，我们将立即进行删除处理， incubate at RT for 1 hour. Rinse with PBS 3x. ABC at RT for 1 hour. Rinse in PBS 3x. ( ABC solution: 5ml PBS with 1 drop A and 1 drop B。

incubate at RT for 1 hr. Wash in PBS 3x Mount with 50% glycerol in PBS. Culture Cell Enzyme Immunostaining for BrdU: Wash plates with in PBS 3x. Put 4%PFA 20 min at RT. Wash with PBS 3x. 0.5% Triton X-100 in PBS， 温馨提示：87%用户都在生物谷APP上阅读， incubateat 4C overnight. Rinse with PBS 3x. Goat Biotinylated anti-Mouse IgG 1:200 in 1% NGS， 1%BSA in PBS。

否则将追究法律责任，其它来源的文章系转载文章， Enzyme Immunostaining for BrdU: Wash frozen sections with PBS 2x. Put them in 0.1% Pepsin in 0.1N HCL (in PBS) at 37C for 50min. Then in 0.3% hydrogen peroxide in PBS。